IMMUNOHISTOCHEMICAL CHARACTERISTICS OF THE GASTRIC MUCOSA IN ALIMENTARY-INDUCED MICROELEMENTOSIS
Keywords:
gastric mucosa, immunohistochemistry, trace element deficiency, magnesium, iron, zinc, selenium, epithelial renewal, inflammation, cellular activity, experimental rats, mucosal injuryAbstract
Alimentary-induced microelement deficiency significantly affects epithelial renewal, immune response, and tissue homeostasis in the gastric mucosa. This study aimed to evaluate immunohistochemical changes in the gastric mucosa of 3-month-old rats under conditions of combined deficiency of magnesium, iron, zinc, and selenium. The experiment was conducted on white outbred rats divided into control and experimental groups. Trace element deficiency was induced through a controlled alimentary model. Gastric tissues were processed using standard histological techniques and stained with hematoxylin and eosin. Immunohistochemical analysis was performed to assess cellular activity, inflammatory response, and regenerative processes. In the control group, the gastric mucosa exhibited normal structural organization, intact epithelial lining, and moderate immunohistochemical activity. In contrast, rats with combined trace element deficiency demonstrated pronounced immunohistochemical alterations. These included increased cellular infiltration in the lamina propria, activation of inflammatory cells, and disruption of epithelial integrity. The findings also indicated impaired epithelial renewal and weakened barrier function of the gastric mucosa. The observed changes suggest the development of chronic mucosal injury associated with microelement imbalance. The results confirm that deficiency of magnesium, iron, zinc, and selenium leads to significant disturbances in cellular activity and immune response in the gastric mucosa. Immunohistochemical analysis provides important insights into the mechanisms of tissue damage and adaptive responses under micronutrient deficiency.
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